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Emfret D200說(shuō)明書(shū)

產(chǎn)品詳情

Cat.No.:XX D200

抗原： 小鼠血小板活化的雙色分析

描述： 該產(chǎn)品提供了一種即用型工具，用于通過(guò)流式細(xì)胞術(shù)確定小鼠血小板的整聯(lián)蛋白αIIbβ3（GPIIb / IIIa）活化和α-顆粒分泌（P-選擇蛋白表達(dá)）。

克隆： Wug.E9 JON / A.

同種型： 大鼠IgG2b / IgG1

形成： FITC PE

應(yīng)用： 流式細(xì)胞術(shù)

尺寸： 2 x 1.0毫升; 200次測(cè)試

價(jià)錢： 505.00美元

Two-color analysis of mouse platelet activation PE- and FITC- labeled antibodies

小鼠血小板活化PE和FITC標(biāo)記抗體的雙色分析

# D200 

克隆/亞型：Jon/A/大鼠(Wistar)IgG2b(1ml-200試驗(yàn))

內(nèi)容：Pe-和FITC標(biāo)記的免疫球蛋白在20 mm Tris緩沖液中加入137 mm NaCl、0.5%BSA和0.09%(w/v)Sodium azide。

大小：2x1.0ml/200試驗(yàn)僅供研究使用，不用于診斷或治療用途。這種產(chǎn)品不是醫(yī)療器械。

該產(chǎn)品提供了一個(gè)現(xiàn)成的工具，以確定整合素aIIbb3(GPIIb/IIIa)的激活和無(wú)粒細(xì)胞分泌(P-選擇素表達(dá))在一次分析中小鼠血小板。

特異性：PE標(biāo)記的jon/A抗體(emfret#m023-2)選擇性地結(jié)合小鼠整合素aIIb 3 1(GPIIb/IIIa，CD 41/CD 61)的高親和力構(gòu)象。 f 135 kDa AIIB鏈和90 kDa b3鏈.整合素aIIbb3是纖維蛋白原、血管性假血友病因子、纖維連接蛋白和維甲酸的血小板受體，介導(dǎo)血小板粘附和凝血功能。 環(huán)境2。整合素aIIbb3的激活依賴構(gòu)象變化，因此Jon/APE的結(jié)合依賴于細(xì)胞外游離鈣。

FITC標(biāo)記的戊型E9抗體(emfret#M130-1)與小鼠P-選擇素(CD62P)反應(yīng)，是一種屬于粘附分子3選擇素家族的140 kDa單鏈多肽。P-選擇素 在血小板的a-顆粒和內(nèi)皮細(xì)胞的Weibel-Palade小體中表達(dá)。細(xì)胞活化后，P-選擇素被轉(zhuǎn)運(yùn)到表面膜上.P-選擇素介導(dǎo)粘附 活化血小板對(duì)中性粒細(xì)胞和單核細(xì)胞的影響。血小板表面P-選擇素的表達(dá)是A顆粒分泌的標(biāo)志.

制備和保存：用蛋白G-Sepharose層析法從雜交瘤細(xì)胞培養(yǎng)上清液中純化抗體.抗體與FITC或R-生理紅蛋白(PE)un結(jié)合。 在宜條件下。這些解決方案沒(méi)有未結(jié)合的熒光團(tuán)。將產(chǎn)品保存在4°C未稀釋，避免長(zhǎng)時(shí)間暴露在光線下。從裝運(yùn)之日起穩(wěn)定一年。別凍著。 在使用前，請(qǐng)將同等體積的A抗體(Jona-PE)和B抗體(WUg.E9-FITC)混合在混合管中。這種混合物可以在4℃下儲(chǔ)存幾個(gè)星期。

用途：抗體的制備是為流式細(xì)胞術(shù)的應(yīng)用而優(yōu)化的。建議用抗體A和B混合液中的10μl對(duì)~(106)血小板或~0.5x106細(xì)胞進(jìn)行染色。 25升泰羅德-Hepes緩沖液含1mm CaCl 2的體積。激動(dòng)劑應(yīng)與染色抗體一起加入。室溫下孵育15分鐘，加入400毫升，停止反應(yīng)。 在30分鐘內(nèi)對(duì)樣品進(jìn)行PBS分析。請(qǐng)注意，孵育時(shí)間、緩沖液條件或抗體濃度的變化可能會(huì)影響結(jié)果的質(zhì)量。

警告：Sodium azide是一種可逆的氧化代謝抑制劑；因此，含有這種防腐劑的抗體制劑不得用于細(xì)胞培養(yǎng)或動(dòng)物體內(nèi)注射。 。Sodium azide可通過(guò)洗滌染色細(xì)胞或平板結(jié)合抗體或在無(wú)Sodium azide緩沖液中透析可溶性抗體來(lái)去除。

10 M腺苷對(duì)25例胎肝緩沖液(1mm CaCl 2)小鼠血小板106枚活化整合素aIIb 3和P-選擇素表達(dá)的檢測(cè) 二磷酸(ADP)或0.2U/ml凝血酶，用10μl A抗體和B抗體直接染色15 min。樣品用400 lPBS填充，直接分析。普拉蒂萊 TS采用FSC/SSC特性。注：ADP是一種相對(duì)較弱的激動(dòng)劑，它刺激(可逆)整合素aIIbb3的激活，但實(shí)際上沒(méi)有P-選擇素的表達(dá)。相反，凝血酶 是一種*的血小板激動(dòng)劑，可誘導(dǎo)強(qiáng)而不可逆的整合素aIIb 3活化和P-選擇素的表達(dá)。

References: 1. Bergmeier W, Schulte V, Brockhoff G, Bier U, Zirngibl H, Nieswandt B. (2002) Flow cytometric detection of activated mouse integrin alphaIIbbeta3 with a novel monoclonal antibody. Cytometry 1;48:80-6. 2. Phillips DR, Charo IF, Scarborough RM. (1990) GPIIb-IIIa: the responsive integrin. Cell. 65(3):359-62. 3. Bird MI, Foster MR, Priest R, et al. (1997) Selectins: physiological and pathophysiological roles. Biochem Soc Trans. 25(4):1199-206 4. Bouchard BA, Tracy PB. (2001) Platelets, leukocytes, and coagulation. Curr Opin Hematol. 8(5):263-9

emfret授權(quán)書(shū)：

EMFRET Analytics—專注脈管系統(tǒng)抗體

    德國(guó)EMFRET公司專注心血管和血液系統(tǒng)研究用抗體研發(fā)和生產(chǎn)，供應(yīng)的抗體包括清除血液中血小板、流式細(xì)胞術(shù)鑒定分析小鼠血小板表面糖蛋白，EMFRET公司的抗體不僅適用于流失分析、WB檢測(cè)，還可用于免疫組化/免疫熒光及免疫共沉淀檢測(cè)。

Cat.No. Antigen Clone Isotype Form Applications Size

C301 Antibodies for Mouse Plaet Depletion polyclonal non-immune rat immunoglobulins Rat IgG purif. negative control for #R300 0.5 mg

D200 Two-Color Analysis of Mouse Plaet Activation Wug.E9 JON/A Rat IgG2b/IgG1 FITC PE flow cytometry 2 x 1.0 ml; 200 tests

M011-0 GPVI JAQ1 Rat IgG2a purif. IP, IHC, WB 0.1 mg

M011-1 GPVI JAQ1 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M021-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b purif. IP, IHC, block 0.5 mg

M021-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M022-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.A1 Rat IgG1 purif. IP, IHC 0.5 mg

M023-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) JON/A Rat IgG2b PE FC (activ. form) 1.5 ml; 300 tests

M024-0 Integrin alphaIIb (GPIIb, CD41) MwReg30 Rat IgG1 purif. IP, IHC, generation of Fab fragments 6 mg

M025-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a purif. IP, IHC 0.5 mg

M025-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M025-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a PE FC, IHC 1.5 ml; 300 tests

M025-3 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a DyLight 649 FC, IHC 1.5 ml; 300 tests

M030-0 Integrin beta3 chain (GPIIIa, CD61) Luc.A5 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M031-0 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b purif. IP, IHC, WB 0.5 mg

M031-1 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M040-0 GPIbalpha (CD42b) Xia.G5 Rat IgG2b purif. IP, IHC 0.5 mg

M040-1 GPIbalpha (CD42b) Xia.G5 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M040-2 GPIbalpha (CD42b) Xia.G5 Rat IgG2b PE FC, IHC 1.5 ml; 300 tests

M040-3 GPIbalpha (CD42b) Xia.G5 Rat IgG2b DyLight 649 FC, IHC 1.5 ml; 300 tests

M041-0 GPIbalpha (CD42b) Xia.H10 Rat IgG2a purif. FC, IHC 0.5 mg

M041-1 GPIbalpha (CD42b) Xia.H10 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M042-0 GPIbalpha (CD42b) Xia.G7 Rat IgG1 purif. IP, IHC, WB 0.5 mg

M042-1 GPIbalpha (CD42b) Xia.G7 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M043-0 GPIbalpha (CD42b) Xia.B2 Rat IgG2a purif. IP, IHC, block 0.5 mg

M043-1 GPIbalpha (CD42b) Xia.B2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M050-0 GPIbbeta (CD42c) Xia.C3 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M050-1 GPIbbeta (CD42c) Xia.C3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M051-0 GPIX (CD42a) Xia.B4 Rat IgG1 purif. IP, IHC 0.5 mg

M051-1 GPIX (CD42a) Xia.B4 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M060-0 GPV (CD42d) Gon.C2 Rat IgG1 purif. IP, IHC 0.5 mg

M060-1 GPV (CD42d) Gon.C2 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M061-1 GPV (CD42d) Gon.G6 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M070-0 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b purif. IP, IHC, block 0.5 mg

M070-1 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M071-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b purif. IP, IHC 0.5 mg

M071-1 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M075-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1, Sam.G4, Sam.H9 Rat IgG2b/IgG1 purif. IP, IHC 0.5 mg

M080-0 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 purif. IP, IHC 0.5 mg

M080-1 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M110-0 CD9 Nyn.H3 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M110-1 CD9 Nyn.H3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M120-0 CD31 (PECAM-1) Pec.H3 Rat IgG1 purif. IP, IHC, WB 0.5 mg

M130-0 CD62P (P-selectin) Wug.E9 Rat IgG1 purif. IP, IHC 0.5 mg

M130-1 CD62P (P-selectin) Wug.E9 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M130-2 CD62P (P-selectin) Wug.E9 Rat IgG1 PE FC, IHC 1.5 ml; 300 tests

P140-1 fibrinogen polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests

P150-1 von Willebrand Factor (vWF) polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests

P180-1 negative control polyclonal Rabbit IgG FITC FC, IHC 1.0 ml; 200 tests

P190-1 negative control polyclonal Rat IgG FITC FC, IHC 1.0 ml; 200 tests

P190-2 negative control polyclonal Rat IgG PE FC, IHC 1.0 ml; 200 tests

P190-3 negative control polyclonal Rat IgG DyLight 649 FC, IHC 1.0 ml; 200 tests

R300 Antibodies for Mouse Plaet Depletion polyclonal anti-GPIb alpha Rat IgG purif. plaet depletion 0.5 mg

X488 Antibodies for In Vivo Mouse Plaet Labeling anti - GPIbbeta derivative Rat IgG DyLight 488 in vivo 100 µg

X649 Antibodies for In Vivo Mouse Plaet Labeling anti - GPIbbeta derivative Rat IgG DyLight 649 in vivo 100 µg

貨號(hào) 抗原類型 克隆方式 表型 產(chǎn)品形式 應(yīng)用 規(guī)格

C301 清除小鼠血小板 polyclonal non-immune rat immunoglobulins Rat IgG 純化 清除小鼠血小板 0.5 mg

D200 雙色分析血小板活性 Wug.E9 JON/A Rat IgG2b/IgG1 FITC、PE 流式細(xì)胞術(shù) 2 x 1.0 ml; 200 tests

M011-0 GPVI JAQ1 Rat IgG2a 純化 IP, IHC, WB 0.1 mg

M011-1 GPVI JAQ1 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M021-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b purif. IP, IHC, block 0.5 mg

M021-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.H4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M022-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.A1 Rat IgG1 purif. IP, IHC 0.5 mg

M023-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) JON/A Rat IgG2b PE FC (activ. form) 1.5 ml; 300 tests

M024-0 Integrin alphaIIb (GPIIb, CD41) MwReg30 Rat IgG1 purif. IP, IHC, generation of Fab fragments 6 mg

M025-0 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a purif. IP, IHC 0.5 mg

M025-1 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M025-2 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a PE FC, IHC 1.5 ml; 300 tests

M025-3 Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) Leo.F2 Rat IgG2a DyLight 649 FC, IHC 1.5 ml; 300 tests

M030-0 Integrin beta3 chain (GPIIIa, CD61) Luc.A5 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M031-0 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b purif. IP, IHC, WB 0.5 mg

M031-1 Integrin beta3 chain (GPIIIa, CD61) Luc.H11 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M040-0 GPIbalpha (CD42b) Xia.G5 Rat IgG2b purif. IP, IHC 0.5 mg

M040-1 GPIbalpha (CD42b) Xia.G5 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M040-2 GPIbalpha (CD42b) Xia.G5 Rat IgG2b PE FC, IHC 1.5 ml; 300 tests

M040-3 GPIbalpha (CD42b) Xia.G5 Rat IgG2b DyLight 649 FC, IHC 1.5 ml; 300 tests

M041-0 GPIbalpha (CD42b) Xia.H10 Rat IgG2a purif. FC, IHC 0.5 mg

M041-1 GPIbalpha (CD42b) Xia.H10 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M042-0 GPIbalpha (CD42b) Xia.G7 Rat IgG1 purif. IP, IHC, WB 0.5 mg

M042-1 GPIbalpha (CD42b) Xia.G7 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M043-0 GPIbalpha (CD42b) Xia.B2 Rat IgG2a purif. IP, IHC, block 0.5 mg

M043-1 GPIbalpha (CD42b) Xia.B2 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M050-0 GPIbbeta (CD42c) Xia.C3 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M050-1 GPIbbeta (CD42c) Xia.C3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M051-0 GPIX (CD42a) Xia.B4 Rat IgG1 purif. IP, IHC 0.5 mg

M051-1 GPIX (CD42a) Xia.B4 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M060-1 GPV (CD42d) Gon.C2 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M061-1 GPV (CD42d) Gon.G6 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M070-0 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b purif. IP, IHC, block 0.5 mg

M070-1 Integrin alpha2 chain (CD49b, GPIa) Sam.G4 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M071-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b purif. IP, IHC 0.5 mg

M071-1 Integrin alpha2 chain (CD49b, GPIa) Sam.C1 Rat IgG2b FITC FC, IHC 1.5 ml; 300 tests

M075-0 Integrin alpha2 chain (CD49b, GPIa) Sam.C1, Sam.G4, Sam.H9 Rat IgG2b/IgG1 purif. IP, IHC 0.5 mg

M080-0 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 purif. IP, IHC 0.5 mg

M080-1 Integrin alpha5 chain (CD49e) Tap.A12 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M110-0 CD9 Nyn.H3 Rat IgG2a purif. IP, IHC, WB 0.5 mg

M110-1 CD9 Nyn.H3 Rat IgG2a FITC FC, IHC 1.5 ml; 300 tests

M120-0 CD31 (PECAM-1) Pec.H3 Rat IgG1 purif. IP, IHC, WB 0.5 mg

M130-0 CD62P (P-selectin) Wug.E9 Rat IgG1 purif. IP, IHC 0.5 mg

M130-1 CD62P (P-selectin) Wug.E9 Rat IgG1 FITC FC, IHC 1.5 ml; 300 tests

M130-2 CD62P (P-selectin) Wug.E9 Rat IgG1 PE FC, IHC 1.5 ml; 300 tests

P140-1 fibrinogen polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests

P150-1 von Willebrand Factor (vWF) polyclonal Rabbit IgG FITC FC, IHC 1.5 ml; 300 tests

P180-1 negative control polyclonal Rabbit IgG FITC FC, IHC 1.0 ml; 200 tests

P190-1 negative control polyclonal Rat IgG FITC FC, IHC 1.0 ml; 200 tests

P190-2 negative control polyclonal Rat IgG PE FC, IHC 1.0 ml; 200 tests

P190-3 negative control polyclonal Rat IgG DyLight 649 FC, IHC 1.0 ml; 200 tests

R300 Antibodies for Mouse Plaet Depletion polyclonal anti-GPIb alpha Rat IgG purif. plaet depletion 0.5 mg

X488 Antibodies for In Vivo Mouse Plaet Labeling anti - GPIbbeta derivative Rat IgG DyLight 488 in vivo 100 µg

X649 Antibodies for In Vivo Mouse Plaet Labeling anti - GPIbbeta derivative Rat IgG DyLight 649 in vivo 100 µg

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