英文名：ICG maleimide
ICG-MAL
中文名：吲哚菁綠-馬來酰亞胺
CAS：2143933-81-5
分子式: C51H56N4O6S
分子量: 853.09
外 觀：綠色粉末
溶解度：二氯甲烷
純 度：95%+
結構式：
ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and emission maximum at 807 nm (10% v/vDMSO/PBS). Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm).
The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.
It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.
近紅外熒光染料Near IRDyes激發和發射波長和顏色圖：
ICG NHS ester的NHS可以和蛋白、多肽或抗體上面的NH2發生反應鏈接到一起。
ICG-maleimide的馬來酰亞胺可以和蛋白、多肽、小分子或抗體上的SH巰基反應連接。
ICG-Amine的NH2可以和蛋白、多肽小分子上面的NHS反應連接起來。
ICG和PEG鏈接可在體內長循環。
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ICG衍生物產品：
ICG NHS ester 近紅外染料吲哚菁綠-琥珀酰亞胺酯
ICG Vinylsulfone 吲哚菁綠-乙烯基砜
ICG isothiocyanate 吲哚菁綠-異硫氰酸酯
ICG Carboxlaic acid ICG-COOH
ICG-Maleimide/ICG-MAL/CAS:2143933-81-5/吲哚菁綠-馬來酰亞胺
ICG amine ICG-NH2 氨基修飾吲哚菁綠
ICG thiol ICG-SH 巰基修飾吲哚菁綠
ICG dichlorotriazine 吲哚菁綠標記的二氯三嗪
ICG Hydrazide ICG-HZ 吲哚菁綠-酰肼
ICG alkyne ICG-alk 吲哚菁綠修飾炔基
ICG Avidin ICG-Avidin 功能化ICG修飾親和素
ICG Biotin ICG-Biotin吲哚菁青-生物素
